The tetracycline resistance determinants of RP1 and Tnl721: nucleotide sequence analysis
Open Access
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (17) , 6089-6105
- https://doi.org/10.1093/nar/11.17.6089
Abstract
Nucleotide sequences of the homologous tetracycline resistance (tet) determinants of plasmid RP1 and transposon Tn1721 have been determined. Two open reading frames of divergent polarity have been assigned to a regulatory gene (tetR) and a gene encoding a resistance protein (tetA). The intercistronic region contains appropriate regulatory and transcription signals. The tetR gene can code for a protein of 216 araino acids (deduced mol.wt. 23,288) and the tetA gene for a protein of 399 amino acids (deduced raol. wt. 42,205). Based on the deduced amino acid sequence, the tetA proteins of RP1/Tn1721 are 78% homologous with that of pBR322 and 45% homologous with that of TnlO. We conclude that a single tetA gene mediates resistance in each of these tet determinants.Keywords
This publication has 17 references indexed in Scilit:
- Directed mutagenesis method for analysis of mutagen specificity: application to ultraviolet-induced mutagenesis.Proceedings of the National Academy of Sciences, 1983
- Nucleotide sequence of the Tn10 encoded tetracycline resistance geneNucleic Acids Research, 1983
- Nucleotide sequences at the ends of the mercury resistance transposon, Tn501Nucleic Acids Research, 1980
- Heterogeneity of tetracycline resistance determinantsPlasmid, 1980
- In vitro and in vivo products of E. coli lactose permease gene are identicalNature, 1980
- Characterisation of Tn1721, a new transposon containing tetracycline resistance genes capable of amplificationMolecular Genetics and Genomics, 1979
- Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322Published by Cold Spring Harbor Laboratory ,1979
- Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivativesMolecular Genetics and Genomics, 1978
- Construction and characterization of new cloning vehicle. II. A multipurpose cloning systemGene, 1977
- λ represser regulates the switch between PR and Prm promotersNature, 1976