Rat Serum “Bound” Insulin: In Vivo Biologic Effects in Rats

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Abstract
Intravenous administration of partially purified preparations of rat serum bound insulin into hypohysectomized rats, produced hypoglycemia and stimulated the incorporation of glucose carbon into the glycogen of muscle and adipose tissue and into the fat of the adipose tissue of these animals. Intraperitoneal injections of rat bound insulin into intact, fed rats also stimulated the incorporation of glucose carbon into the glycogen of muscle and adipose tissue and into the fat of the adipose tissue. Rat bound insulin, lite human, was less effective in stimulating glycogen synthesis than fat synthesis on adipose tissue, as compared with crystalline insulin. The in vivo biologic effects of rat bound insulin in rats correspond to those produced by human bound insulin or cyrstalline bovine insulin. The properties of rat bound insulin appear to be similar to those of human bound insulin. Rat bound insulin, like human, was obtained from sera by resin adsorption and elution, was biologically active in vivo, inactive on isolated hemldlaphragm, unless adipose tissue extract (ATE) was added into the incubating medium, and unreactive with anti-insulin antisera.