Isolation of temperature-sensitive mutants of R plasmid by in vitro mutagenesis with hydroxylamine
- 1 September 1976
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 127 (3) , 1561-1563
- https://doi.org/10.1128/jb.127.3.1561-1563.1976
Abstract
Temperature-sensitive [Escherichia coli] mutants were isolated from a tetracycline resistance plasmid, pSC101, after mutagenesis with hydroxylamine. They were divided into 3 classes according to their growth characteristics. They include mutants defective in the replication of plasmid DNA and strains having a mutation in the drug resistance gene.This publication has 5 references indexed in Scilit:
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- Transformation of Salmonella typhimurium by Plasmid Deoxyribonucleic AcidJournal of Bacteriology, 1974
- Construction of Biologically Functional Bacterial Plasmids In VitroProceedings of the National Academy of Sciences, 1973
- Isolation and characterization of a temperature-sensitive amber suppressor mutant of Escherichia coli K12Molecular Genetics and Genomics, 1973
- Mutagenic treatment of double- and single-stranded DNA phages T4 and S13 with hydroxylamineVirology, 1968