Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations
Open Access
- 1 October 1989
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 185 (1) , 205-210
- https://doi.org/10.1111/j.1432-1033.1989.tb15103.x
Abstract
Adenylate cyclase catalytic subunits from Neurospora crassa membranes may interact with regulatory factors from membranes of bovine retinal rod outer segments (pretreated with N-ethylmaleimide), reconstituting a heterologous system which, in the presence of light, is catalytically active in assay mixtures containing MgATP. Maximal activation was observed at 550 nm. Transducin-depleted retinal membranes were not capable of reconstituting the heterologous light-stimulated adenylate cyclase system. Addition of a transducin preparation to depleted membranes restored the reconstitution capacity of these membranes. A similar heterologous adenylate cyclase system was reconstituted with Neurospora and mouse retinal whole membranes (pretreated with N-ethylmaleimide). Membranes from mice suffering photoreceptor degeneration (rd homozygotes) did not reconstitute an heterologous adenylate cyclase system.This publication has 35 references indexed in Scilit:
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