A specific translocator for oxaloacetate transport in chloroplasts
- 3 December 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 178 (1) , 15-19
- https://doi.org/10.1016/0014-5793(84)81230-2
Abstract
Evidence is provided that oxaloacetate is taken up by chloroplasts from maize and spinach leaves by a high‐affinity carrier. Apparent K m values for oxaloacetate are about 45 and 9 μM, respectively. Uptake was measured by following [4‐14C]oxaloacetate reduction in illuminated chloroplasts. With maize chloroplasts the rates of uptake were sufficient to account for photosynthesis. This carrier had a very low affinity for malate and 2‐oxoglutarate. Ratios of the K i, for malate or 2‐oxoglutarate to the K m for oxaloacetate were about 150 and 50, respectively.Keywords
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