Brefeldin A Prevents Uncovering but not Phosphorylation of the Recognition Marker in Cathepsin D
- 1 January 1990
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler
- Vol. 371 (2) , 567-574
- https://doi.org/10.1515/bchm3.1990.371.2.567
Abstract
Brefeldin A (BFA) has been shown to inhibit transiently the subcellular transport of cathepsin D (Oda and Nishimura (1989) Biochem. Biophys. Res. Commun. 163, 220-225). We studied the effect of this antibiotic on processing of the phosphorylated oligosaccharides in cathepsin D in human promonocytes U937. In the presence of the drug the phosphorylation of cathepsin D precursor continued at a diminished rate. The phosphorylated oligosaccharides in cathepsin D comprised mono- and bisphosphorylated forms. The relative amounts of the two species were not changed in the presence of BFA. The uncovering of the phosphate groups and the proteolytic processing of the phosphorylated precursor were abolished. In an in vitro assay the uncovering enzyme, N-acetylglucosamine-1-phosphodiester N-acetylglucosaminidase was not inhibited by BFA. We suggest that this drug interrupts the traffic between the compartments containing N-acetylglucosaminyl phosphotransferase and N-acetylglucosamine-1-phosphodiester N-acetylglucosaminidase.This publication has 16 references indexed in Scilit:
- Microtubule-dependent retrograde transport of proteins into the ER in the presence of brefeldin a suggests an ER recycling pathwayCell, 1990
- Targeting and processing of glycophorins in murine erythroleukemia cells: use of brefeldin A as a perturbant of intracellular traffic.Proceedings of the National Academy of Sciences, 1989
- Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: Evidence for membrane cycling from Golgi to ERCell, 1989
- Evidence that luminal ER proteins are sorted from secreted proteins in a post-ER compartment.The EMBO Journal, 1988
- Effects of Differentiation-Inducing Agents on Synthesis, Maturation and Secretion of Cathepsin D in U937 and HL-60 CellsBiological Chemistry Hoppe-Seyler, 1987
- Novel blockade by brefeldin A of intracellular transport of secretory proteins in cultured rat hepatocytes.Journal of Biological Chemistry, 1986
- Brefeldin A, a specific inhibitor of intracellular translocation of vesicular stomatitis virus G protein: Intracellular accumulation of high-mannose type G protein and inhibition of its cell surface expression.Agricultural and Biological Chemistry, 1985
- Processing and Transport of Lysosomal Enzymes in Human Monocyte Line U937Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1984
- Phosphorylated oligosaccharides in lysosomal enzymes: identification of alpha-N-acetylglucosamine(1)phospho(6)mannose diester groups.Proceedings of the National Academy of Sciences, 1980
- Two species of lysosomal organelles in cultured human fibroblastsCell, 1979