Analysis of Serum Bile Acids by Gas-Liquid Chromatography Equipped With 63Ni-Electron Capture Detector
- 1 January 1971
- journal article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 9 (1) , 53-58
- https://doi.org/10.1093/chromsci/9.1.53
Abstract
The trifluoroacetylated methyl esters of bile acids in quantities as small as 0.1 ng were quantitatively analyzed by a gas-liquid chromatograph equipped with a 63Ni-electron capture detector. The different numbers of modified hydroxyl groups in bile acids gave different responses to an electron capture detector, but quantitative analysis was still carried out with high reproducibility using calibration curves of the various bile acids. The characteristics of a 63Ni-electron capture detector (63Ni-ECD) were studied and it was shown that the pulse method was far more suited for the microanalysis than the direct current method. A procedure for the microanalysis of the bile acid derivatives was established and applied to the serum bile acids in samples of serum as small as 0.2 ml.Keywords
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