ISOLATION OF TRANSPLANTABLE ERYTHROLEUKEMIA-CELLS FROM MICE INFECTED WITH HELPER-INDEPENDENT FRIEND MURINE LEUKEMIA-VIRUS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 58  (2) , 244-254
Abstract
The Friend virus complex contains a helper-independent retrovirus, F-MuLV [Friend murine leukemia virus], and a replication-defective retrovirus, SFFV [spleen focus forming virus]. Murine erythroleukemia cell lines (MEL) were isolated previously from the leukemic tissues of mice infected with such stocks of Friend virus complex. Since it was shown that F-MuLV or SFFV can induce a lethal erythroproliferative disease, it has been unclear which of the properties of conventional MEL cells were causally related to the infection of these cells by F-MuLV and which properties were related to the infection of these cells by SFFV. The helper-independent component of the Friend virus complex, F-MuLV clone 57, which was recently purified by molecular cloning, was studied. A method is reported for the reproducible isolation of a new type of murine erythroleukemia cell that can be isolated from mice infected with F-MuLV clone 57. These cells were designated as 57TP-1, indicating their origin from mice infected with F-MuLV clone 57 and their characteristics as transplantable erythroleukemia cells. Upon s.c., i.p. or i.v. inoculation of these cells, 57TP-1 cells grow in the hematopoietic organs of the recipient mice. No s.c. or i.p. nodules occur. F-MuLV and a Friend MCF virus can be detected in these cells but no evidence of SFFV can be found.

This publication has 14 references indexed in Scilit: