Ligand binding to ferrocytochrome c at high pH
- 1 May 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 147 (2) , 335-341
- https://doi.org/10.1042/bj1470335
Abstract
Ferrocytochrome c has been shown to bind two molecules of CO at pH 14. The second CO is thought to be bound only when the cytochrome c molecule is denatured, and once bound appears to be spectrally silent. Insolubilization of native cytochrome c prevents the binding of the second CO molecule. A scheme is proposed to explain these observations based on evidence from static titrations and flash-photolysis experiments, use of carboxymethyl cytochrome c and insoluble cytochrome c, and use of cyanide instead of CO as a ligand.Keywords
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