Identification ofin vitro metabolites of 3-ethyl-2,6-dimethyl-4H-pyrido(1,2a)pyrimidin-4-one

Abstract
(2-14C)3-Ethyl-2,6-dimethyl-4H-pyrido(1,2a)pyrimidin-4-one is metabolized in vitro by liver microsomes to at least 12 metabolites. The metabolites were isolated and purified by thin-layer chromatography and high-performance liquid chromatography and identified by mass spectrometry and nuclear magnetic resonance spectrosopy. The mostly mono- and dihydroxylated isomers were distinguished on the bases of their electron ionization mass spectra and the metastable daughter ion spectra of selected ions.

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