The Role of the H Locus in Heterokaryosis in Rhizoctonia solani

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Abstract
R. solani grew as button-like colonies on a medium containing L-sorbose and polygalacturonic acid. Consequently, 3 auxotrophs were detected and isolated from basidiospores irradiated with UV light. These were used to determine the function of the 2 closely linked genes (together called H factor) that control heterokaryon formation in R. solani. When different monoauxotrophs were paired on minimal agar, strains with either the same H factor (H=) or different H factors (H.noteq.) yielded heterokaryons. The H.noteq. heterokaryons were considerably more stable than the H= heterokaryons. When fruited, H.noteq. heterokaryons showed strict outcrossing between the 2 paired strains. The H= heterokaryons yielded progeny from both outcrossed and selfed basidia. The H locus functions like incompatibility loci in other basidiomycetes and controls the stability and outbreeding of the heterokaryon.

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