Purification of Immunosuppressive Factors Extracted From Bovine Spleen (Lymphoid Chalone)

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Abstract
A low MW immunosuppressive factor FA which can reduce the blastic transformation capacity of lymphoid cells from treated mice was characterized. It was prepared from a bovine spleen acetone powder and associated partly with high MW carriers in the form of an active complex characterized previously as part of a ''lymphoid chalone'' fraction. FA may be obtained by selective ultrafiltration of F followed by P-2 Biogel chromatography of the ultrafiltrate. Thymidine, deoxyinosine and deoxycytidine were identified as the major constituents of FA by mass spectrometry, UV absorption data and TLC. None of these nucleotides has the biological activity of FA.