Studies on the Perfused Rat Liver: II. Effect of Glucagon on Gluconeogenesis

Abstract
In vitro studies using the isolated perfused liver from fasted rats have demonstrated increased glucose production in the presence of glucagon. The incorporation of C14-labeled alanine into glucose was increased, as was production of urea, suggesting a primary effect of glucagon in stimulating gluconeogensis. Time studies showed this effect to occur in 10-15 mins. Incorporation of pyruvate and lactate C14 was also increased, as was that of HC-14-O3.