Regional changes in cardiac structural proteins in myocardial infarction. Biochemical and histologic correlates.

Publisher Website
Google Scholar
Abstract
Quantitative analysis of structural proteins from a minute amount of myocardial tissue was performed from 10 .mu. thick cardiac tissue slices weighing 2-5 mg by the extraction in glycerol solution and by sodium dodecyl sulfate (SDS) gel electrophoresis. These changes were compared with the histologic alterations in the striated structure of the adjacent cardiac slices in the experimental myocardial infarction in the dog. Approximately 69 .mu.g of structural proteins were obtained from 1 mg of the normal heart muscle. In the central portions of the myocardial infarction, reductions in myosin heavy chain (HC), light chain (LC) 1 and .alpha.-acetinin were observed at 12-24 h after the coronary occlusion followed by the decrease in myosin LC 2 at 48 h. Those changes became intense at 72 h to 7 days, but restored gradually at 14-28 days. Alterations in the striated structure of cardiac muscle fibers of the adjacent tissue slices were found simultaneously with the changes in structural proteins. At 12-24 h after the coronary ligation increase in eosinophilia and overstretch of cross-striation were observed. The findings of coagulation necrosis, loss of striation, fragmentation, swelling of A-band, etc., of the infarcted fibers were markedly observed at 48 h to 7 days, but the histologic restoration of cardiac fibers was found with the recovery of the infarcted tissue at 14-28 days after the coronary ligation. Changes in the compositions of structural proteins corresponded well to the alterations in the striated structure in chronology and in quantity.

This publication has 0 references indexed in Scilit: