Microbiological Studies of Plaque in Artificial Fissures Implanted in Human Teeth

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Abstract
Microbial colonization was studied in artificial Mylar ‘fissures’ implanted for 1, 3 and 7 days in a molar tooth in each of six subjects who maintained an excellent oral hygiene. The microflora of the ‘fissure’ content was studied by performing total microscopic counts as well as viable counts on various media. The samples contained mainly streptococci. The number of lactobacilli was low but tended to increase with time. The absence of spirochetes and the low numbers of spirilla, fusiforms and filaments support the hypothesis that fissures represent an ecological system differing from that on the smooth surfaces of the teeth.

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