Effective method for obtaining long nucleotide chains on partially complementary templates Processed bovine γ-interferon gene obtained from human γ-interferon gene
Open Access
- 5 June 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 249 (2) , 379-382
- https://doi.org/10.1016/0014-5793(89)80663-5
Abstract
The method of obtaining the bovine γ‐interferon gene by means of simultaneous multidirected mutagenesis of the human γ‐interferon gene is presented. The first strand of the bovine γ‐interferon gene was obtained by ligation of synthetic oligonucleotides, using the cDNA of human γ‐interferon, cloned in the single‐stranded phage M13mpl9 as a template. The second strand was synthesized using a large fragment of E. coli DNA‐polymerase 1. The double‐stranded gene was then treated by restriction nucleases and cloned in a pUC‐ 18 derived vector. The primary structure was confirmed by sequencing.Keywords
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