Hehoglobin Brest [β127(H5)Gln→LYS] a new Unstable Human Hemoglobin Variant Located at the α1β1 Interface with Specific Electrophoretic Behavior

Abstract
Hb Brest [.beta.127(H5)Gln .fwdarw. Lys] is a new unstable variant located at the .alpha.1.beta.1 interface at the same position as Hb Complutense [.beta.127(H5)Gln .fwdarw. Glu]. In each of these, the substitution produces a distinct alteration in charge, yet both variants move with Hb A in conventional electrophoresis. This peculiar electrophoretical behavior may be due to the molecular position of the modified residue, which is deeply buried inside the tetramer.

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