Growth of genetically engineered Pseudomonas aeruginosa and Pseudomonas putida in soil and rhizosphere

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Abstract
The ability of a bacterium to grow in soil and rhizosphere exists because the bacterium possesses genes for this growth. Using recombinant DNA techniques, it may be possible to add a specific gene to a bacterium and determine whether this gene is favorable or deleterious to growth of this bacterium. In this manner, the ecological significance of the gene can be assessed. The objective of our experiment was to determine the effect of the addition of a recombinant plasmid containing a pgl A gene encoding for an α-1,4-endopolygalacturonase from Pseudomonas solanacearum on the growth of Pseudomonas aeruginosa and Pseudomonas putida in soil and rhizosphere. The recombinant plasmid pJE8 containing the pgl A gene on a 30-kb P. solanacearum DNA fragment in the 20-kb cos-mid vector pLAFR3 and the vector alone were conjugally transferred into both P. aeruginosa and P. putida.