Cytoplasmic membrane fluidity measurements on intact living cells ofBacillus subtilis by fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene
- 1 January 1988
- journal article
- research article
- Published by Springer Nature in Folia Microbiologica
- Vol. 33 (1) , 1-9
- https://doi.org/10.1007/bf02928006
Abstract
A method for determination of membrane fluidity (microviscosity) inBacillus subtilis cytoplasmic membrane underin vivo conditions is described. The membranes were labelled with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene during the exponential phase of growth. Fluorescence anisotropy values were determined as soon as 4 min after termination of the cultivation. Fluorescence anisotropy measurements were carried out in an intact cell suspension having absorbanceA as high as 0.2-0.3 (corresponding to a cell concentration of 100-300/nL).Keywords
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