Reversion from Erythromycin Dependence in Escherichia coli: Strains Altered in Ribosomal Sub-unit Association and Ribosome Assembly
- 1 May 1988
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 134 (5) , 1251-1263
- https://doi.org/10.1099/00221287-134-5-1251
Abstract
A mutant of Escherichia coli dependent on erythromycin for growth spontaneously gives erythromycin-independent strains with altered or missing ribosomal proteins. Strains with defects in ribosome assembly were sought and obtained from among these revertants. Two organisms in which ribosomal protein L9 is altered and absent respectively have 70S ribosomes whose dissociation into sub-units is particularly sensitive to pressures generated during centrifuging. The mutant that lacks protein L19 also accumulates ribosome precursor particles during exponential growth as do others including mutants that lack proteins S20 or L1. These strains also show unbalanced synthesis of RNA and so will be useful in investigating both the pathways and the regulation of ribosome assembly.This publication has 1 reference indexed in Scilit:
- Mutants of Escherichia coli lacking ribosomal protein L1Journal of Molecular Biology, 1981