Heart Protection by Cardioplegic Solutions Containing Oxyhemoglobin Pretreated by Carbontetrachloride and Freeze-Drying with Sucrose
- 1 January 1992
- journal article
- Published by Taylor & Francis in Biomaterials, Artificial Cells and Immobilization Biotechnology
- Vol. 20 (2-4) , 709-720
- https://doi.org/10.3109/10731199209119707
Abstract
A technologically improved variant of native stroma–free oxyhemoglobin (SFH) pretreated by carbontetrachlo-ride and freeze-drying with 240 mM sucrose were reconstituted in a properly diluted ionic solution to reach the final concentration of 66 g oxyhemoglobin/L, osmolality 290–320 m0sm and pH 7.4. Cardioplegia of isolated rat heart was induced and maintained by this solution without recirculation for 3 h at 20°C prior to heterotopic allotransplantation of the graft. Evaluation of the survival and performance of each graft after 24 h and extent of tissue necroses indicated that the given standardly produced SFH variant ensured reproducible heart preservation from ischemic and reperfusion injury similarly as did the renown crystalloid cardioplegic solution Custodiol.Keywords
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