Transcriptional regulation of a Purkinje cell‐specific gene through a functional interaction between RORα and RAR

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Abstract
Background: The orphan nuclear receptor RORα is highly expressed in the Purkinje cells of the cerebellum during the postnatal development of brain. A recent observation has been made that the RORα gene is disrupted in staggerer mice—which show a cell‐autonomous defect in the development of the Purkinje cells. Results: In order to understand the functions of RORα in cerebellar development, I attempted to identify its target genes. Transient expression study demonstrated that transcription of the Purkinje cellprotein‐2 (Pcp‐2) gene is activated by RORα,which binds as a monomer to a single half‐site motif (RORE) within the promoter region. Its transcription was also activated by retinoic acid receptor (RAR) which binds as a heterodimer with RXR to a retinoic acid responsive element (RARE) in the downstream region. Interestingly, the RORα‐mediated transcription is further activated synergistically by RAR. Conclusion: That the Pcp‐2 gene is a targetof RORα, and is suggested that its transcription is also regulated by RAR.

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