Comparison of immunological reactivity of polygalacturonases from different fungi
- 1 January 1988
- journal article
- research article
- Published by Taylor & Francis in Giornale botanico italiano
- Vol. 122 (1) , 1-6
- https://doi.org/10.1080/11263508809429387
Abstract
Homogeneous endo-polygalacturonases (PGs) from Aspergillus niger and Fusarium moniliforme were used to prepare rabbit antisera. IgG were purified from antisera by Protein A-sepharose chromatography. Purified IgG were tested against endopolygalacturonases from Sclerotium rolfsii, Sclerotinia sclerotiorum, Rhizoctonia solani, Rhizoctonia fragariae and Fusarium oxysporum. Double immunodiffusion tests, ELISA tests and Western blottings showed that all the enzymes react with both antisera suggesting that a basic PG structure is mantained among the different fungi.Keywords
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