Increasing the variability of Lycopersicon peruvianum Mill. by protoplast fusion with Petunia hybrida L.

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Abstract
Somatic hybridization of Lycopersicon peruvianum and Petunia hybrida was carried out to transfer cytoplasmic male sterility from Petunia to Lycopersicon. Cytological, morphological and biochemical analyses were performed to characterize the regenerated plants. Two regenerated plants, R3 and R6, were male sterile. R3 possessed chromosomes morphologically similar to those of both parental types. Leaf morphologies of these two plants and a third plant, R7, were intermediate between the two parents. The stability of RUBPCase was verified during parental plant development and after in vitro culture. Plant R7 presented a new form of the large subunit of RUBPCase.