Antigenic Differences Between the Sperm of Different Inbred Strains of Mice

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Abstract
Pooled mice sperm, chiefly from C57 [male][male], was suspended in Locke''s soln. or in serum to be absorbed, and then injd. into B alb C [female][female]. Each injn. contained 10 million to 30 million sperm. After 3 injns. there were 4 days rest and then 4 to 7 repetitions of injns. and rest. The mice were killed 7 to 14 days after the last injn. The initial titer of the antisera ranged from 1:24 to 1:512. Absorptions were performed for 1 hr. or more at room temp., followed by an overnight period in the refrigerator and later 1/2 hr. at room temp. Agglutination tests in hanging drops or vials were read under the microscope every 15 to 30 min. after addn. of sperm to the antisera. Of 9 antisera tested, 4 were +, 4 were [long dash], and 1 was doubtful. Anti-C57 sperm serum absorbed with C sperm clearly differentiated betw. C57 and C sperm, strongly agglutinated C57 and left C free-swimming. The results with anti-F1 (C57 X P) sperm serum indicate a 2d distinguishing antigen or group of antigens in the P sperm.

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