Systematic study of the substituted active C‐terminus of hirudin
- 5 December 1999
- journal article
- Published by Wiley in Chemical Biology & Drug Design
- Vol. 54 (6) , 480-490
- https://doi.org/10.1034/j.1399-3011.1999.00132.x
Abstract
Hirudin, a potent clinical thrombin inhibitor from Hirudo medicinalis, consists of 65 amino acids in a single chain. In this paper, we systematically synthesize a series of C-terminal (desulfo hirudin45-65) peptides substituted by 20 natural L-amino acids via the Multipin method. The resulting peptide library is subsequently screened using an alpha-thrombin-mediated fibrinogen clotting assay and alpha-thrombin-induced amidolytic hydrolysis assay.Keywords
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