DETECTION OF ANTIBODIES IN HUMAN-SERUM USING TRIMELLITYL-ERYTHROCYTES - DIRECT AND INDIRECT HEMAGGLUTINATION AND HEMOLYSIS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 39  (2) , 470-476
Abstract
Utilizing trimellityl-erythrocytes (TM-E), antibodies were detected in sera of 7 workers with trimellitic anhydride(TMA)-induced airway syndromes by direct hemagglutination, indirect hemagglutination with anti-human Ig[immunoglobulin]G, IgA or IgM or by hemolysis. Detectable levels of antibody were obtained with all 3 methods. The most sensitive technique was indirect hemagglutination using anti-IgG. When added as an inhibitor, TM-human serum albumin produced a 10- to 800-fold reduction in titers. TM-ovalbumin of similar epitope density was less inhibitory and sodium trimellitate the least inhibitory on a molar basis. All of the assays using haptenized human red cells were capable of detecting anti-TM antibodies in Rhesus monkeys whose airways had been exposed to TMA. These assays are useful for detecting anti-TM antibodies and may be adapted to demonstrate antibodies induced against other inhaled haptens in sera of environmentally exposed individuals or in animal models of such exposure.