Effects of sodium n-butyrate on alpha-fetoprotein and albumin secretion in the human hepatoma cell line PLC/PRF/5
Open Access
- 1 March 1985
- journal article
- research article
- Published by Springer Nature in British Journal of Cancer
- Vol. 51 (3) , 357-363
- https://doi.org/10.1038/bjc.1985.47
Abstract
The in vitro effects of sodium n-butyrate (butyrate) on the growth, morphology and secretion of alpha-fetoprotein (AFP) and albumin by the human heptoma cell line PLC/PRF/5 were studied. Butyrate caused a marked reduction in the growth rate, colony forming efficiency in soft agar and de novo synthesis of DNA as well as remarkable morphological changes including cell enlargement, flattening and a decreased number of nucleoli. Secretion of AFP was reduced during culture with butyrate, while that of albumin was increased. The requirement of de novo protein synthesis for the increase in albumin and decrease of AFP by butyrate was demonstrated by inhibition studies with cycloheximide. These results suggest that butyrate caused the hepatoma cells to acquire in vitro properties that are considered to be more consistent with normal liver cells.Keywords
This publication has 23 references indexed in Scilit:
- Sodium butyrate produces concordant expression of “early placental” alkaline phosphatase, pregnancy-specific beta1-glycoprotein and human chorionic gonadotropin beta-subunit in a newly established uterine cervical cancer cell line (SKG-IIIa)International Journal of Cancer, 1983
- Phenotypic changes induced in the human thymic all cell line HPB-all by an ingenol ester, milliaminInternational Journal of Immunopharmacology, 1983
- Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoproteinJournal of Immunological Methods, 1981
- Enhanced albumin production by malignantly transformed hepatocytes during in vitro exposure to dimethylsulfoxideBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1980
- Anti-proliferative properties of DL-α-difluoromethyl ornithine in cultured cells. A consequence of the irreversible inhibition of ornithine decarboxylaseBiochemical and Biophysical Research Communications, 1978
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Processing of Newly Synthesized Histone MoleculesScience, 1975
- Butyric acid, a potent inducer of erythroid differentiation in cultured erythroleukemic cellsCell, 1975
- Serum alpha-fetoprotein, albumin, and gamma-G-globulin in the human conceptus.Journal of Clinical Investigation, 1966
- ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESISProceedings of the National Academy of Sciences, 1964