Effect of a C/EBP gene replacement on mitochondrial biogenesis in fat cells
Open Access
- 2 August 2004
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 18 (16) , 1970-1975
- https://doi.org/10.1101/gad.1213104
Abstract
CCAAT/enhancer-binding proteins, C/EBPα and C/EBPβ, are required for fat cell differentiation and maturation. Previous studies showed that replacement of C/EBPα with C/EBPβ, generating the β/β alleles in the mouse genome, prevents lipid accumulation in white adipose tissue (WAT). In this study, β/β mice lived longer and had higher energy expenditure than their control littermates due to increased WAT energy oxidation. The WAT of β/β mice was enriched with metabolically active, thermogenic mitochondria known for energy burning. The β/β allele exerted its effect through the elevated expression of the G protein α stimulatory subunit (Gαs) in WAT. Gαs, when overexpressed in fat-laden 3T3-L1 cells, stimulated mitochondrial biogenesis similar to that seen in the WAT of β/β mice, and effectively diminished the stored lipid pool.Keywords
This publication has 40 references indexed in Scilit:
- Simple flow cytometric method used to assess lipid accumulation in fat cellsJournal of Lipid Research, 2004
- Obesity Wars: Molecular Progress Confronts an Expanding EpidemicPublished by Elsevier ,2004
- Insulin signalling and the regulation of glucose and lipid metabolismNature, 2001
- Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based TherapiesTissue Engineering, 2001
- The -Adrenergic Receptors and the Control of Adipose Tissue Metabolism and ThermogenesisRecent Progress in Hormone Research, 2001
- CCAAT/Enhancer-binding Protein α Is Required for Transcription of the β3-Adrenergic Receptor Gene during AdipogenesisJournal of Biological Chemistry, 2001
- C/EBPβ, When Expressed from the C/ebpα Gene Locus, Can Functionally Replace C/EBPα in Liver but Not in Adipose TissueMolecular and Cellular Biology, 2000
- Hyperproinsulinaemia in obese fat/fat mice associated with a carboxypeptidase E mutation which reduces enzyme activityNature Genetics, 1995
- Epididymal white adipose tissue after cold stress in rats II. Mitochondrial changesJournal of Ultrastructure and Molecular Structure Research, 1988
- G PROTEINS: TRANSDUCERS OF RECEPTOR-GENERATED SIGNALSAnnual Review of Biochemistry, 1987