The Effect of Feeding Different Protein-Free Diets on the Recovery and Amino Acid Composition of Endogenous Protein Collected from the Distal Ileum and Feces in Pigs

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Abstract
Nine barrows with an average initial weight of 60 kg were fitted with simple T-cannulas at the distal ileum. The animals were fed four different protein-free diets according to an incomplete latin square design. Diet 1 (control diet) consisted of 79.7% cornstarch, 10% sucrose, 3% Alphafloc (a source of cellulose), 3% canola oil and a vitamin-mineral premix. Diets 2, 3 and 4 contained, respectively, 4% pectin, an additional 7% cellulose and an additional 10% canola oil, each included at the expense of cornstarch. Feces were collected during 3 d following a 7-d adaptation period. Thereafter, digesta were collected during two 24-h periods with a 24-interval between periods. The pigs were fed 800 g of feed twice at 0800 and 2000. Added pectin increased (P < .05) the recovery of endogenous protein in ileal digesta from 19.8 diet (diet 1) to 24.0 g per kg dry matter intake. This increment was largely due to increases (P < .05) in glycine and proline from 1.9 to 2.4 and from 6.2 to 8.4 g per kg dry matter intake, respectively. In feces, only added cellulose increased (P < .05) excretion of endogenous protein (8.4 vs 11.1 g/kg DM intake) and of most amino acids. Including additional fat did not affect the quantity of endogenous protein and amino acids recovered in ileal digesta or feces. Small but significant differences (P < .05) were observed in the amino acid composition of endogenous protein recovered in ileal digesta when the different protein-free diets were fed. The amino acid composition of endogenous protein in feces was relatively constant (P > .05). Copyright © 1989. American Society of Animal Science . Copyright 1989 by American Society of Animal Science

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