A New Relaxed Mutant of Escherichia coli with an Altered 50S Ribosomal Subunit
- 1 September 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (9) , 3465-3469
- https://doi.org/10.1073/pnas.71.9.3465
Abstract
A new relaxed mutant called rel C has been isolated from a rel A + /rel A + partial diploid strain. The rel C mutant is unable to synthesize ppGpp or pppGpp in vivo in response to amino acid starvation or in vitro , but can synthesize these nucleotides in a shift-down. Rel C maps near rif . Studies in vitro demonstrate the lesion to be probably in one of the 50S ribosomal proteins that can be removed by 1.0 M LiCl.Keywords
This publication has 19 references indexed in Scilit:
- Localization of the Stringent Protein of Escherichia coli on the 50S Ribosomal SubunitProceedings of the National Academy of Sciences, 1974
- Thermolability of the stringent factor in rel mutants of Escherichia coliJournal of Molecular Biology, 1973
- Codon specific, tRNA dependent in vitro synthesis of ppGpp and pppGpp.1973
- Accumulation and turnover of guanosine tetraphosphate in Escherichia coliJournal of Molecular Biology, 1972
- MSI and MSII made on Ribosome in Idling Step of Protein SynthesisNature, 1972
- Ribosomal ProteinsEuropean Journal of Biochemistry, 1971
- Two Compounds implicated in the Function of the RC Gene of Escherichia coliNature, 1969
- Diazomethane as the active agent in nitrosoguanidine mutagenesis and lethalityMolecular Genetics and Genomics, 1968
- Mutations and Genetics Concerned With the RibosomeCold Spring Harbor Symposia on Quantitative Biology, 1966
- A GENETIC LOCUS FOR THE REGULATION OF RIBONUCLEIC ACID SYNTHESISProceedings of the National Academy of Sciences, 1961