Sulphhydryl and disulphide stainings of subepidermal hyaline bodies

Publisher Website
Google Scholar
Abstract
The localization of SH-groups and disulfide [S-S] bonds in subepidermal hyaline bodies was examined using histochemical techniques with the specific fluorescent thiol reagent [N-(7-dimethylamino-4-methylcoumarinyl) maleimide] (DACM). In lichen planus patients, hyaline bodies showed S-S but not SH staining, suggesting that cross-linking of SH-groups occurred and favored their derivation from epidermal cells. In lupus erythematosus and dermatomyositis, there were S-S positive hyaline bodies, considered of epidermal origin and S-S negative ones. The latter may consist of dermal components, since no dermal components were stainable with DACM. DACM staining helped determine the origin of hyaline bodies.