N‐tosyl‐L‐phenylalanylchloromethane reacts with cysteine 81 in the molecule of elongation factor Tu from Escherichia coli
- 27 December 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 150 (2) , 485-488
- https://doi.org/10.1016/0014-5793(82)80795-3
Abstract
Elongation factor EF-Tu from Escherichia coli was labelled with N-[14C]tosyl-L-phenylalanylchloromethane, digested with trypsin and the peptides obtained separated by HPLC. The only radioactive peak recovered corresponded to tryptic peptide containing residues 75-98. Sequencing of the peptide by automated Edman degradation identified cysteine 81 as the site of N-tosyl-L-phenylalanylchloromethane modification. These results confirm the importance of this residue for the interaction with aminoacyl-tRNAs.Keywords
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