The MTCY428.08 Gene of Mycobacterium tuberculosis Codes for NAD + Synthetase
Open Access
- 15 June 1998
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 180 (12) , 3218-3221
- https://doi.org/10.1128/jb.180.12.3218-3221.1998
Abstract
The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD + synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S -transferase fused and His 6 -tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD + synthetase.Keywords
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