Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins
- 1 November 2004
- journal article
- research article
- Published by Springer Nature in Biotechnology Letters
- Vol. 26 (22) , 1731-1737
- https://doi.org/10.1007/s10529-004-3745-3
Abstract
Developing seeds from Triticum aestivum (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR. Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.Keywords
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