DNA Ligase in Pea (Pisum sativumL.) Seedlings: Changes in Activity during Germination and Effects of Deoxyribonucleotides

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Abstract
Daniel, P. P. and Bryant, J. A. 1988. DNA ligase in pea (Pisum sativum L.) seedlings: changes in activity during germination and effects of deoxyribonucleotides.—J. exp. Bot. 39: 481–486. Measurable amounts of ‘soluble’ and chromatin-bound DNA bgase are present in the embryo axis at least as early as 8 h after the onset of imbibition. Between 8 h and 48 h the activity of the chromatin-bound form increases nearly 10-fold, whilst the soluble activity increases 6-fold. There is then a slight decline in the chromatin-bound activity between 48 h and 72 h, whilst the soluble activity continues to increase. Both forms of ligase arc markedly inhibited by dTTP (but not by other deoxyribonucleoside triphosphates); the soluble form is significantly more sensitive to dTTP than the chromatin-bound form.