Roles of Arg231 and Tyr284 of Thermus thermophilus isocitrate dehydrogenase in the coenzyme specificity
Open Access
- 28 November 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 355 (2) , 171-172
- https://doi.org/10.1016/0014-5793(94)01191-5
Abstract
The coenzyme binding site of isocitrate dehydrogenase from Thermus thermophilus was analyzed by site‐directed mutagenesis. The mutation analysis revealed that Arg231 and Tyr284 are involved in the discrimination between NAD and NADP, suggesting that these two residues interact with 2′‐phosphate group of NADP.Keywords
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