ESTABLISHMENT OF CELL-LINES OF UVEAL MELANOMA - METHODOLOGY AND CHARACTERISTICS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 25  (11) , 1284-1299
Abstract
Continuous cell lines (6) were established from choroidal and ciliary body melanomas. These lines were maintained in culture for at least 100 in vitro population doublings for period over 1 yr. They were established initially using a human diploid fibroblast strain MRC-5 as a feeder layer. Cells were grown in Ham''s F-12 medium containing fetal bovine and horse sera and supplemented with glucose, cholera toxin and epidermal growth factor. Culture doubling times ranged from 72-96 h; cloning efficiencies ranged from 1-5% in the absence of a feeder layer. Cell lines (6) were studied in detail by EM and all were found to have evidence of melanosomes and/or premelanosomes. The cell morphology was characteristic of melanomas as defined by the Callender classification, with cell types ranging from spindle A to epithelioid. Karyotypic studies revealed the presence of only human chromosomes with modal numbers ranging from 48-54 in the different lines.

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