Liquid Chromatographic Analysis of Doxazosin in Human Serum with Manual and Robotic Sample Preparation
- 1 November 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 26 (11) , 570-573
- https://doi.org/10.1093/chromsci/26.11.570
Abstract
A specific method for the determination of the antihypertensive drug doxazosin in human serum is described. The method utilizes the related drug prazosin as an internal standard and is based on a simple extraction scheme followed by analysis by reversed-phase ion suppression high-performance liquid chromatography (HPLC) on an alumina-based column with fluorescence detection. The method is completely automated with a flexible robotic system for the analysis of drugs in biological fluids. The robotic automation of the method allows a 20% increase in the sample throughput and the savings of about 7 man-hours a day. Both the manual and robotic procedures yield precise quantitative results over the therapeutically relevant concentration range of 0.5 to 20 ng/mL of serum.This publication has 1 reference indexed in Scilit:
- Quantitative analysis of doxycycline and related substances by high-performance liquid chromatographyJournal of Chromatography A, 1985