Liquid Chromatographic Analysis of C1-C10Carbonyls

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Abstract
Retention times, response factors, uv-visible spectra and chemical ionization (CI) mass spectra are presented for 2,4-dinitrophenylhyrazine (DNPH) derivatives of C1–C10 carbonyls analysed by liquid chromatography (LC) with uv detection, LC-diode array detection and on-line LC-mass spectrometry using C18 columns and ucetonitrile-water eluent. The forty two carbonyls studied include aliphatic aldehydes (saturated and unsaturated), aliphatic ketones (saturated and unsaturated), aromatic aldehydes, hydroxycarbonyls, oxo-acids and dicarbonyls. A correlation was obtained between response factors (R1 = peak height for I μg/mL carbon) i > and retention times (tR), log Rf = (-1.33 ± 0.05) log tR + (2.98 ± 0.05), R = 0.985, n = 28. LC-diode array and LC-MS analysis have been used to show that %bT-hydroxycarbonyls yield the DNPH derivatives of the corresponding dicarbonyls. The results are of diagnostic value to identify and measure carbonyls at low-concentrations in complex environmental mixtures including ambient air.