Stopped-flow-photometric and kinetic-fluorimetric methods for the determination of thyroid hormones in tablets

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Abstract
The catalytic effect of thyroxine and triiodothyronine on the reaction between cerium(IV) and arsenic(III) was studied by using photometric and fluorimetric measurements. The kinetic-photometric studies were based on measurement of the decrease in absorbance of cerium(IV), whereas the rate of formation of fluorescent cerium(III) was measured in the kinetic-fluorimetric studies. A modular stopped-flow system was coupled to a spectrophotometer in order to develop stopped-flow methods for the determination of thyroxine and triiodothyronine. The proposed methods were compared and applied to the determination of these hormones in pharmaceutical preparations.

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