Enzyme immunoassay for the detection of levamisole in meat and milk
- 1 January 1994
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 119 (12) , 2705-2706
- https://doi.org/10.1039/an9941902705
Abstract
Ovine polyclonal antisera were raised to aminolevamisole to develop an enzyme immunoassay for levamisole. The assay has been validated in milk and in 100 g l–1 muscle homogenate. The limit of detection is 1 µg l–1. In milk the inter-assay relative standard deviation (sr) is 6% at 10 µg kg–1 and the intra-assay sr value 7.2%. In tissue homogenate the inter-assay sr value is 12.7% at 5 µg kg–1 and the intra-assay sr value is 3.8%. Total incubation time is 1 h.Keywords
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