The Respiration of the Pea Plant. Oxidation of Hexose Phosphate and Pentose Phosphate by Cell-free Extracts of Pea Leaves.

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Abstract
Cell-free extracts of 11-15 day pea leaves oxidized fructose-l,6-diphosphate (FDP), fructose-6-phosphate, glucose-6-phosphate, 6-phosphogluconate, and ribose-5-phosphate in the presence of triphosphopyridine nucleo-tide (TPN). Glucose, gluconic acid, ribose, ribonic acid-5-phos-phate were inactive. The oxidations are TPN-specific. Dialyzed extracts are inactive but regain activity by addition of [mu]g quantities of riboflavin or riboflavin phosphate and mg quantities of adenosine triphosphate. FDP is oxidized in the presence of diphosphopyridine nucleotide (DPN) and arsenate. When TPN is substituted for DPN, arsenate stimulates oxidation only slightly. The paper indicates the presence in pea leaf tissue of a "hexose-monophosphate shunt" which closely resembles that found in animal tissue and yeast.