Controlled Staining of Autoradiographs
- 1 January 1958
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 33 (5) , 221-223
- https://doi.org/10.3109/10520295809111853
Abstract
The preparation of autoradiographs in which the tissue and the emulsion are in permanent register is often complicated by staining after the photographic image has been developed and fixed. While general oversight methods can be satisfactory, controlled, specific staining can be obtained with most basic dyes when the pH is properly regulated. The reactivity of the gelatin is suppressed at a pH of 4 or slightly below whereas nuclei, ergastoplasm, cartilage, mast cells, mucus, etc. stain readily. Basic fuchsin,. 05% at pH 3.5-4.0 in dilute (1:10) McLlvaine buffer, is recommended. The final preparation contrasts in color and transparency with the black, opaque silver grains.Keywords
This publication has 1 reference indexed in Scilit:
- The stripping-film technique of autoradiographyThe International Journal of Applied Radiation and Isotopes, 1956