A gene probe for TEM type beta-lactamases
- 1 July 1985
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 28 (1) , 154-156
- https://doi.org/10.1128/aac.28.1.154
Abstract
A restriction fragment of plasmid pBR322 bearing the TEM-1 beta-lactamase structural gene was electroeluted from agarose gels after digestion with EcoRI and HinfI. The 1-kilobase fragment was 32P-labeled and used to examine genetic relationships with nucleic acids encoding seven other beta-lactamase classes. The probe hybridized only with TEM-2 and OXA-2 class plasmids.Keywords
This publication has 13 references indexed in Scilit:
- Mechanisms of Bacterial Resistance to Antimicrobial Agents, with Particular Reference to Cefotaxime and Other β-Lactam CompoundsClinical Infectious Diseases, 1982
- A physical and genetic map of the IncN plasmid R46Plasmid, 1981
- Acquisition by Escherichia coli of plasmid-borne β-lactamases normally confined to Pseudomonas sppPlasmid, 1979
- The distribution of β-lactamase genes on plasmids found in PseudomonasPlasmid, 1979
- Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322Published by Cold Spring Harbor Laboratory ,1979
- Emergence of Antibiotic Resistance In Hospitals, 1935-1975Clinical Infectious Diseases, 1979
- Partial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K.Proceedings of the National Academy of Sciences, 1978
- The β-lactamases of Gram-negative bacteria and their rôle in resistance to β-lactam antibioticsJournal of Antimicrobial Chemotherapy, 1976
- Reduction in the rate of DNA reassociation by sequence divergenceJournal of Molecular Biology, 1973
- The relation of resistance transfer factors to the F-factor (sex-factor) ofEscherichia coliK12Genetics Research, 1966