Germ Line Basis for Antibody Diversity: Immunoglobulin V H - and C H -gene Frequencies Measured by DNA·RNA Hybridization
- 1 January 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (1) , 99-103
- https://doi.org/10.1073/pnas.71.1.99
Abstract
The reiteration frequency for mouse immunoglobulin V(H)-genes and C(H)-genes has been directly estimated by hybridization of purified MOPC 315 alpha-chain mRNA with a vast excess of mouse DNA. A biphasic C(o)t curve resulted. The low C(o)t transition (C(o)t(1/2) about 1.5) was interpreted as hybridization to V(H)-genes and the high C(o)t transition (C(o)t(1/2) about 10(3)) as hybridization to C(H)-genes. These values correspond to about 5000 V(H)-genes and less than 8 C(H)-genes. This germ-line content of V(H)-genes is sufficient to account for antibody diversity given a comparable set of V(L)-genes. Minimal-gene models are invalidated and there is no need to invoke somatic generators of diversity.Keywords
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