The N- and c-terminal amino acid sequences of the heavy chain from a pathological human immunoglobulin IgG

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Abstract
The heavy chain of a pathological human immunoglobulin IgG and also the Fd fragment have been isolated. No free a-amino group was present on either and the N-terminal sequence of both has been identified as pyrolid-2-one-5-carbonylvalylthreonine. Splitting at the 4 methionine residues of the heavy chain with cyanogen bromide gave 5 fractions. The fraction from the C-terminal end of the chain was isolated in high yield and the amino acid sequence was His-Glu-Ala-Leu-His-Asp (NH2)-His-Tyr-Thr-Glu(NH2)-Lys-Ser-Leu-Ser-Leu-Ser-Pro-Gly These results give strong support to the view that the heavy chain of immunoglobulin is a single peptide chain.