The effect of monoiodoacetate on the aerobic metabolism of ox retina in vitro

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Abstract
The rate of oxygen consumption of ox retina incubated in Ringer bicarbonate solution is increased by the addition of glucose but not by that of pyruvate. When retina is incubated in Ringer phosphate solution the reverse is the case, for in the presence of glucose the rate of O2 consumption is slightly depressed, although the initial rate is maintained for a longer period; addition of pyruvate causes a small rise in O2 consumption. Addition of monoiodoacetate at a concentration of m[image] causes only a small reduction in the amount of oxygen used during incubation in Ringer phosphate solution, although lactic acid production is strongly inhibited. During incubation in Ringer bicarbonate solution containing glucose O2 consumption is inhibited by 40% in a concentration of 0.1 m[image] iodoacetate; an increase in concentration of iodoacetate to 1.0 m[image] does not bring about a significant further reduction in O2 uptake.