Involvement of the p38 mitogen‐activated protein kinase pathway in tissue inhibitor of metalloproteinases‐1‐induced erythroid differentiation
Open Access
- 21 November 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 485 (2-3) , 117-121
- https://doi.org/10.1016/s0014-5793(00)02210-9
Abstract
We examined the role of the mitogen‐activated protein (MAP) kinase pathway in tissue inhibitor of metalloproteinases‐1 (TIMP‐1)‐mediated cellular effects in a human erythroleukemic cell line UT‐7. We show that TIMP‐1 induced both UT‐7 cell erythroid differentiation and proliferation and tyrosine phosphorylation of many intracellular proteins. Using a panel of phosphospecific antibodies, we also demonstrate that phosphorylation of the p38 and c‐Jun N‐terminal kinases is increased by TIMP‐1 whereas phosphorylation of extracellular signal‐regulated kinase 1/2 is not induced. Moreover, inhibition of the p38 activity by SB203580 significantly reduces erythroid differentiation induced by TIMP‐1, suggesting that the p38 MAP kinase pathway is involved in TIMP‐1‐induced erythroid differentiation.Keywords
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