Ribonucleotide Reductase Activity in Hydroxyurea-Resistant Herpesvirus Replication

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Abstract
The role of an altered ribonucleotide diphosphate reductase activity in the resistance of equine herpesvirus type 1 to hydroxyurea was demonstrated. A 28% increase in total CDP reductase was observed by 6 h PI [post-infection] and the enzyme activity of EHV-1 [equine herpesvirus type-1 infected mouse fibroblast L-M] cells differed from that of mock-infected cells in several properties. Infected cell activity did not require ATP for in vitro activity, exhibited a lower apparent Km and demonstrated a decreased sensitivity to hydroxyurea added directly to the reaction mixtures. The altered CDP reductase activity was first detected at 3-4 h PI, the time of initiation of viral DNA synthesis.

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